Physiological and Pharmacological Influences on Thyroxine to 3,5,3′-Triiodothyronine Conversion and Nuclear 3,5,3′-Triiodothyronine Binding in Rat Anterior Pituitary
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منابع مشابه
Physiological and pharmacological influences on thyroxine to 3,5,3'-triiodothyronine conversion and nuclear 3,5,3'-triiodothyronine binding in rat anterior pituitary.
Our recent in vivo studies have suggested that intrapituitary l-thyroxine (T(4)) to 3,5,3'-triiodo-l-thyronine (T(3)) conversion with subsequent nuclear binding of T(3) is an important pathway by which circulating T(4) can inhibit thyrotropin release. The present studies were performed to evaluate various physiological and pharmacological influences on these two processes in rat anterior pituit...
متن کاملDirect radioimmunoassay of nuclear 3,5,3' triiodothyronine in rat anterior pituitary.
Previous tracer studies have suggested that 5'-monodeiodination of l-thyroxine (T(4)) in anterior pituitary may contribute a substantial portion of specifically bound nuclear 3,5,3' l-triiodothyronine (T(3)) in this tissue in rats. To evaluate this possibility, a radioimmunoassay for nuclear T(3) in individual anterior pituitaries was developed. Animals received [(125)I]T(3) 60 min before remov...
متن کاملSalicylate-induced increases in free triiodothyronine in human serum. Evidence of inhibition of triiodothyronine binding to thyroxine-binding globulin and thyroxine-binding prealbumin.
Addition of sodium salicylate to human serum at concentrations often obtained during aspirin therapy causes 100-200% increases in free triiodothyronine (T(3)) and free thyroxine (T(4)) as estimated by ultrafiltration. The increase in free T(3) was unexpected since previous data had suggested that salicylate inhibits binding of T(4) only to thyroxine-binding prealbumin (TBPA) and that T(3) is no...
متن کاملNuclear triiodothyronine-binding protein: partial characterization and binding to chromatin.
Nuclei were prepared by sucrose sedimentation of liver homogenates from rats given (125)I-labeled triiodothyronine in vivo. The nuclear extract obtained by treatment of the nuclear pellet with 0.4 M KCl contains the [(125)I]triiodothyronine that had been injected in vivo bound to protein(s). The triiodothyronine bound to nuclear protein(s) in vivo does not readily exchange with triiodothyronine...
متن کاملEffects of triiodothyronine and other thyroxine analogues on thyroxine-binding in human serum.
The strong affinity between thyroxine and the specific thyroxine-binding protein (TBP) of human serum (1) is apparent from, a) the inability to remove more than small amounts of thyroxine from the serum by prolonged dialysis (2), and b) the effectiveness with which TBP competes with albumin for available thyroxine in serum, despite its much smaller concentration. Thyroxine bound to TBP is, neve...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1979
ISSN: 0021-9738
DOI: 10.1172/jci109598